Момичета, Т. Милачич ни изпрати следната статия, предполагам представлява интерес за вас. Поставяме е с оригиналния англ. текст:

Gradient centrifugation of frozen spermatozoa for IUI improves pregnancy rates
Milachich, T. ; Petkova, L.; Barov, D.; Timeva, T.; Savova D., Shterev A.
In vitro unit in ObGyn hospital “Dr.Shterev” MC Reproductive Health, Sofia, Bulgaria

(статия четена от Т. Милачич на Балканския конгрес по репродуктивна медицина, Солун, Гърция, 24-26 Септември 2004 г.  под. егидата на членовете на ESHRE)

Intrauterine insemination (IUI) is a part of assisted reproductive technologies and it is less invasive and less expensive, which makes it more preferable by a number of patients.
This retrospective study (January 2001-December 2003) includes 439 IUI in natural unstimulated cycles on total number of 224 subfertile patients: women with husband (IUI-H) insemination  (n = 115, 51.3%) and women with donor frozen-thawed specimen (IUI-D) (n = 109, 48.7%). When the dominant follicle reached 18mm diameter and Oestradiol level >350 pg/ml, HCG (10.000 IU Pregnyl, Organon) was administrated. 36h later the IUI was performed. In this retrospective study two different methods were applied for manipulation of frozen-thawed spermatozoa for IUI-D cycles and in some IUI-H cycle: swim-up (group A) and gradient centrifugation using silane-coated silica particles (group B).
The gestation sac was observed by transvaginal ultrasound and thus the clinical pregnancy rate (PR) was evaluated. The PR per patient was significantly higher in donor insemination group 30.3% (36 ongoing pregnancies/109 patients) versus the husband IUI-H group 20.9% (24 ongoing pregnancies /115 patients) (P<0.05). All IUI were performed in natural cycle, thus no multiple pregnancy occurred. When swim-up was used for frozen-thawed sperm (group A) the PR is significantly lower (26.9%) than in group B (33.3%), when gradient centrifugation was made (P<0.05).
Despite the discouraging data reported world wide on inseminations without stimulation, this method is close to the nature and our results are satisfying. Another reason is to avoid risk of multiple pregnancy and complications. This study was conducted to compare the results of two different techniques for sperm processing on frozen-thawed spermatozoa, used in IUI. It is also shows the need of flexible protocols in order to obtain better quality and concentration of the insemination aliquot, respectively a higher PR comparing to the standardized laboratory protocols.